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Condition:
Factory New
- Weight: 3.13 lb
- Store tubes upright at room temperature (18-25° C)
- Protect tubes from direct light
- Shelf life at 18-25° C is one year from the manufacture date
- This item is non-returnable
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Learn more:
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MFR:
BD
Ships from MFI Medical
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MPN: 362753
- Phone: (877) 232-8995
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Frequently asked questions
Are there various size and anticoagulant options?
Yes, these BD Vacutainer CPT tubes are available in several configurations, offering versatility to meet diverse laboratory requirements for mononuclear cell preparation and subsequent diagnostic assays.
The key options include:
- Anticoagulant: You can choose between two primary formulations based on your specific protocol needs:
- Sodium Heparin
- Sodium Citrate
- Tube Size and Draw Volume: Two different configurations are available based on the required blood sample volume:
- Small: 13 mm x 100 mm tube size with a 4 mL draw volume.
- Standard: 16 mm x 125 mm tube size with an 8 mL draw volume.
Are the CPT tubes a complete, closed system?
Yes, the BD Vacutainer CPT tubes are specifically designed as a fully closed system for mononuclear cell preparation. This key feature allows cell separation to occur entirely within the primary collection tube, which minimizes manual processing steps, reduces variability, and enhances reproducibility.
Each sterile, evacuated glass tube comes pre-filled with all the necessary integrated components for density gradient separation upon centrifugation:
- Anticoagulant: Tubes contain either Sodium Heparin or Sodium Citrate, depending on the configuration selected.
- Density Medium: Tubes contain a liquid density medium (Ficoll Hypaque Solution) necessary for the separation process.
- Inert Gel Barrier: This gel migrates during centrifugation to create a stable physical barrier, effectively isolating the concentrated layer of mononuclear cells suspended in plasma from the heavier erythrocytes and granulocytes.
Is the inert gel barrier highly effective?
Yes, the inert gel barrier is a highly effective and crucial component of the BD Vacutainer CPT system. It is engineered to streamline and standardize the isolation of mononuclear cells (MNCs) for in vitro diagnostic examinations.
This integrated separation mechanism provides significant advantages:
- Fully-Closed System Separation: The gel allows cell separation to occur directly within the primary, closed collection tube, minimizing manual handling and reducing processing variability.
- Stable Physical Barrier: During centrifugation, the inert gel migrates to form a stable, physical separation layer.
- Clean Isolation: This barrier precisely separates the desired concentrated MNC suspension (in plasma) from the unwanted heavier components, such as erythrocytes and granulocytes, ensuring high-quality sample preparation.
Are the results consistently reliable?
Yes, the BD Vacutainer CPT is designed to provide reproducible results, which significantly decreases the processing variability often associated with manual mononuclear cell separation techniques.
Are specific storage requirements needed to maintain sample integrity?
To ensure optimal performance and maintain the stability of the density medium, please adhere to these storage guidelines:
- Temperature: Store upright at a controlled room temperature of 18-25° C.
- Environment: Keep the tubes protected from direct light to prevent degradation of the contents.
- Longevity: These tubes have a shelf life of one year from the date of manufacture when stored correctly.
Can I transport samples in the original collection tube?
Yes. The BD Vacutainer CPT system is specifically engineered to allow for the safe transport of concentrated cell suspensions within the original collection vessel. Thanks to the integrated inert gel barrier, which creates a stable physical interface during centrifugation, your samples remain secure and protected from contamination from collection all the way to the point of analysis.
What is the shelf life of the CPT tubes?
The shelf life for the BD Vacutainer Mononuclear Cell Preparation Tubes (CPT) is one year from the date of manufacture.
To ensure the integrity and performance of the tubes, they must be stored according to these specific conditions:
- Temperature: Store upright at room temperature, specifically between 18° C and 25° C.
- Position: Must be stored upright.
- Protection: Must be protected from direct light.
Is a rigorous sterilization process used?
Yes, a rigorous sterilization process is used, ensuring the integrity of this fully-closed system tube for sensitive cell preparation. The BD Vacutainer CPT tubes meet strict international standards for in vitro diagnostic use:
- Sterilization Method: Autoclave technology is utilized.
- Compliance Standard: Sterilization procedures adhere to the international standard ISO 17665-1.
- Sterility Assurance Level (SAL): The tubes achieve a high sterility level of 10⁻⁶ SAL.
Are high-quality glass tubes used?
Yes, the BD Vacutainer CPT tubes are made from sterile, high-quality glass to ensure structural integrity and functional reliability.
These tubes are engineered to support the complex, fully-closed system for cell preparation:
- Material Integrity: The glass provides the necessary structural support for the evacuated tube system and the proper function of the internal components (anticoagulant, density medium, and inert gel barrier).
- High Sterility: The tubes meet rigorous sterilization standards (Autoclave, 10⁻⁶ SAL) and conform to ISO17665-1, guaranteeing a sterile environment for blood collection and mononuclear cell separation.
How many tubes are in each case?
Each case of BD Vacutainer Mononuclear Cell Preparation Tubes [CPT] includes 60 tubes.
BD Vacutainer Mononuclear Cell Prepar...