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Condition:
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Country of Origin: USA
- Weight: 5.2 lb
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Learn more:
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MFR:
Simport Scientific
Ships from MFI Medical
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MPN: T329-1
- Phone: (800) 464-1723
Frequently asked questions
Is the sealing film guaranteed RNAse, DNAse, and DNA-free?
Yes, this sealing film is manufactured by Simport Scientific to meet the stringent purity requirements necessary for sensitive molecular biology procedures, such as PCR and qPCR.
The film is certified free of critical biological contaminants:
- RNAse-free
- DNAse-free
- DNA-free
This robust contamination profile ensures the film will not interfere with your sensitive experiments. Furthermore, the adhesive itself is designed not to interfere with standard cycle reactions, maximizing the reliability of your results.
Does this film offer superior contamination control?
Yes, the Simport Secureseal Thermal Adhesive Sealing Film is specifically engineered for superior contamination control during sensitive thermal cycling procedures like PCR. It actively protects sample integrity through several specialized features:
- Contamination Prevention: Effectively reduces well-to-well contamination and spill-over, while minimizing evaporation and vapor loss.
- Guaranteed Purity: Manufactured to be certified RNAse-free, DNAse-free, and DNA-free, meeting stringent laboratory standards for molecular assays.
- Thermal Stability: The robust 2.0 mil polyolefin film maintains a strong, reliable seal across a wide range, functional from -70°C to +95°C.
- Non-Interfering: The pressure-sensitive acrylate adhesive is chemically inert and does not interfere with cycle reactions.
Is the sealing film highly thermostable?
The Simport Secureseal film is engineered for excellent thermal stability, making it reliable for all sensitive PCR and thermal cycling applications. It maintains its functional integrity across a broad temperature range, from -70°C to +95°C.
This robust thermal stability is crucial for minimizing evaporation and vapor loss, ensuring sample integrity and preventing well-to-well contamination during standard molecular biology workflows.
Does the film have superior thermal stability for PCR?
Yes, the Simport Secureseal film is engineered for highly demanding thermal cycling protocols, including PCR and qPCR. It is constructed to ensure minimal evaporation and cross-contamination.
Key features that guarantee its superior performance in sensitive assays include:
- Wide Thermal Range: The film maintains a secure seal and integrity across a wide temperature spectrum, from -70°C up to +95°C, accommodating all necessary cycling steps.
- High Purity: It is certified RNAse-free, DNAse-free, and DNA-free, which is essential for sensitive nucleic acid work.
- Chemical Compatibility: The 2.0 mil polyolefin film is resistant to DMSO and exhibits low autofluorescence, ensuring it does not interfere with optical readings or chemical reactions.
Is the Simport Secureseal™ Thermal Adhesive Sealing Film resistant to DMSO?
Yes, the Simport Secureseal™ Thermal Adhesive Sealing Film is DMSO resistant, making it suitable for applications involving this solvent.
Does the sealing film have low autofluorescence?
Yes, the Simport Secureseal Thermal Adhesive Sealing Film exhibits low autofluorescence, which helps to minimize background interference during sensitive PCR applications.
How thick is the polyolefin sealing film?
The Simport Secureseal is constructed from a robust 2.0 mil polyolefin film. This thickness is paired with a pressure-sensitive acrylate adhesive, which ensures a reliable seal for sensitive molecular biology procedures, such as PCR, without interfering with the cycle reactions.
What robust materials create this thermal seal?
The Secureseal Thermal Adhesive Sealing Film is constructed from high-purity components designed specifically for sensitive PCR and thermal cycling protocols:
- Film Material: A robust 2.0 mil polyolefin film forms the core. This material is transparent and exhibits low autofluorescence, which is necessary for accurate optical readings during assays.
- Adhesive: A specially formulated pressure-sensitive acrylate adhesive coats the film. This non-interfering adhesive provides a strong, contamination-free barrier that effectively minimizes evaporation and prevents well-to-well cross-contamination.
This construction ensures the film maintains integrity across an extreme thermal range, from -70°C up to +95°C, and is resistant to common laboratory chemicals like DMSO. The film is also certified RNAse, DNAse, and DNA-free.
Does the Secureseal film prevent cross-contamination?
Yes, the Simport Secureseal Thermal Adhesive Sealing Film is specifically engineered to ensure complete sample integrity and prevent well-to-well contamination during sensitive thermal cycling procedures like PCR and qPCR. Its design focuses on creating a robust, reliable seal.
Key features that ensure a secure environment include:
- Contamination Control: The film effectively minimizes vapor loss and evaporation, which are primary causes of cross-contamination and spillover between adjacent wells.
- High Purity Guaranteed: It is manufactured to meet stringent laboratory standards, certified as RNAse-free, DNAse-free, and DNA-free.
- Thermal Stability: The seal remains reliable across a broad temperature range, functional from -70°C up to +95°C, suitable for demanding thermal cycling protocols.
- Chemical Resistance: The film features DMSO resistance and utilizes a non-interfering, pressure-sensitive acrylate adhesive.
Is the sealing film highly thermostable?
Yes, this sealing film is engineered for excellent thermal stability, making it ideal for sensitive PCR and thermal cycling applications. Its robust formulation ensures the film maintains its integrity and provides a secure seal across a wide operational range:
- Minimum Operating Temperature: -70°C (suitable for cryogenic storage)
- Maximum Operating Temperature: +95°C (suitable for thermal cycling)
This stability is crucial for effectively preventing vapor loss and minimizing evaporation during the entire thermal cycling process.